Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (131,835)

Singleplex and Multiplex Bead Based Immunoassays (5,857)

ELISA Substrates and Reagents (2,333)

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136 – 150 of 137,482 results

136

Invitrogen™ Human IgG (Total) Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 10 μL, Serum 10 μL
Assay Sensitivity	1.6 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	1.6 to 100 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Regulatory Status	RUO
Target	Human IgG
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Sample Type	Plasma,Serum
Label or Dye	HRP
Synonym	Immunoglobulin G

137

Invitrogen™ Mouse TNF alpha Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	CLMF,CLMF2,IL-12 B,IMD28,IMD29,NKSF,NKSF2,CLMF,IL-12 A,NFSK,NKSF1,P35
Total Assay Time	24 hrs 30 mins
Sample Volume	Lavage Fluid 100 μL, Plasma 100 μL, Serum 100 μL, Supernatant 100 μL, Tissue Homogenate 100 μL
Assay Sensitivity	8 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	8 to 1,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P06804
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	21926
Sample Type	Lavage Fluid,Plasma,Serum,Supernatant,Tissue Homogenates
Label or Dye	HRP
Synonym	Tumor Necrosis Factor alpha, tnf-a,tnf alpha

138

Invitrogen™ Human SMAD3 (Phospho) [pS423/pS425] InstantOne™ ELISA Kit

ELISA

Pricing & Availability
Specifications

For Use With (Equipment)	Colorimetric Microplate Reader
Gene ID (Entrez)	4088
For Use With (Application)	ELISA

139

Invitrogen™ Mouse IL-10 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	IL-1 beta,Il-1 b
Total Assay Time	24 hrs 30 mins
Sample Volume	Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	32 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	32 to 4,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P18893
Regulatory Status	RUO
Target	IL-10
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,Coating Buffer: 10X PBS ELISA Coating Buffer,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16153
Sample Type	Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin 10, CSIF, Cytokine Synthesis Inhibitory Factor

140

Invitrogen™ Universal Assay Buffer (10X)

Universal Assay Buffer

Pricing & Availability

141

Invitrogen™ Human IgA ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	4.8%
Intraassay CV	3.9
Total Assay Time	1 hrs 30 mins
Sample Volume	Plasma 10 μL, Serum 10 μL
Assay Sensitivity	0.03 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	1.6 to 100 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Regulatory Status	RUO
Target	Human IgA
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3493, 3494
Sample Type	Plasma,Serum
Label or Dye	HRP
Synonym	Immunoglobulin A

142

Invitrogen™ ProcartaPlex™ Human Antibody Isotyping Panel, 7plex

The ProcartaPlex Human Antibody Isotyping Panel 7plex enables the determination of antibody heavy chain subclasses by analyzing 7 protein targets using Luminex xMAP technology.

Pricing & Availability

143

Invitrogen™ ProcartaPlex™ Human Inflammation Panel, 20plex

The ProcartaPlex Human Inflammation Panel 20plex enables the exploration of immune function by analyzing 20 protein targets in a single well using Luminex xMAP technology.

Pricing & Availability

144

Invitrogen™ ProcartaPlex™ Human Th1/Th2 Cytokine Panel, 11plex

The ProcartaPlex Human Th1/Th2 Cytokine Panel 11plex enables the study of the Th1/Th2 response by analyzing 11 protein targets in a single well using Luminex xMAP technology.

Pricing & Availability

145

Invitrogen™ IL-2 Human ProcartaPlex™ Simplex Kit

Ideal solution for assessing multiple protein biomarkers in a single sample. Explore our broad portfolio of pre-configurated panels and combinable single analytes.

Pricing & Availability

146

Invitrogen™ Rat IL-1 beta ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Rat
Conjugate	HRP
Interassay CV	10%
Intraassay CV	10
Gene Symbol	Il1b
Total Assay Time	3 hrs 10 mins
Sample Volume	Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	4.0 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	31.3 to 2,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Accession Number	Q63264
Regulatory Status	RUO
Target	IL-1 beta
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	24494
Sample Type	Serum,Supernatant
Label or Dye	HRP
Synonym	Catabolin, Interleukin-1 beta, IL1F2

147

Invitrogen™ Human IgM Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Assay Sensitivity	15.6 ng/mL
Assay Range	15.6 to 1,000 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Regulatory Status	RUO
Target	Human IgM
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Human IgM for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3507
Sample Type	Plasma,Serum,Supernatant
Synonym	Immunoglobulin M

148

Invitrogen™ Mouse IL-13 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	RATTNF,TNF-alphaa,Tnf
Total Assay Time	24 hrs 30 mins
Sample Volume	Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	4 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	4 to 500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P20109
Regulatory Status	RUO
Target	IL-13
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,eBioscience ELISA/ ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16163
Sample Type	Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-13, IL13

149

Invitrogen™ Rat TNF alpha ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Rat
Conjugate	HRP
Interassay CV	10%
Intraassay CV	5%
Total Assay Time	3 hr 10 min
Sample Volume	Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	11.0 pg/mL
Storage Requirements	2-8°C
Assay Range	39.1-2500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Accession Number	P16599
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	24835
Sample Type	Serum,Supernatant
Label or Dye	Biotin
Synonym	Cachectin, Tumor necrosis factor alpha, DIF

150

Invitrogen™ Mouse PLGF-2 (PGF) ELISA Kit

ELISA

Pricing & Availability
Specifications

Protein Family	Growth Factors & Receptors
Target Species	Mouse
Conjugate	HRP
Intraassay CV	<10
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity	1 pg/mL
Research Discipline	Development
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hrs 20 mins
Assay	Solid-phase sandwich ELISA
Target	PGF
Specificity	The sandwich ELISA antibody pair detects Mouse PLGF-2.
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Label or Dye	HRP
Sufficient For	96 Assays
Calibration Range	1 pg/mL to 400 pg/mL
Interassay CV	<12%
Gene Symbol	Pgf
Storage Requirements	2°C to 8°C
Assay Range	1.64 to 400 pg/mL
Accession Number	P49764
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	18654
Sample Type	Plasma,Serum,Supernatant
Synonym	AI854365, PIGF, Plgf

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