Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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Invitrogen™ ProcartaPlex™ Cell Lysis Buffer
Used to lyse cells under nondenaturing conditions. Invitrogen™ ProcartaPlex™ Cell Lysis Buffer is available in 20mL units that should be stored chilled (for continual use) or frozen (for longer periods of time.) Additional protease inhibitors can be added without any difficulties.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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R&D Systems™ Human IFN-beta Quantikine ELISA Kit
R&D Systems™ Human IFN-beta Quantikine ELISA Kit is designed to measure human IFN-beta in cell culture supernates.
Invitrogen™ Mouse IgG2a Uncoated ELISA Kit with Plates
ELISA
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| Target Species | Mouse |
|---|---|
| Conjugate | HRP |
| Total Assay Time | 24 hr 30 min |
| Sample Volume | Plasma 10 μL, Serum 10 μL, Supernatant 50 μL |
| Assay Sensitivity | 3.90 ng/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 3.90-250 ng/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 45 min |
| Regulatory Status | RUO |
| Target | Mouse IgG2a |
| Kit Contents | Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Immunoglobulin G2a |
Invitrogen™ ProcartaPlex™ Human Immune Monitoring Panel, 65plex
The ProcartaPlex Human Immune Monitoring Panel 65plex enables the study of immune function by analyzing 65 protein targets in a single well using Luminex xMAP technology.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Human IL-17A ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 9.1% |
| Intraassay CV | 7.1 |
| Gene Symbol | Ctla-8,Ctla8,IL-17,IL-17 A,Il17 |
| Total Assay Time | 3 hrs 10 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.5 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 1.6 to 100 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 20 mins |
| Accession Number | Q16552 |
| Regulatory Status | RUO |
| Target | IL-17 A |
| Kit Contents | Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Controls,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3605 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Interleukin 17 A, CTLA8 |
Invitrogen™ ProcartaPlex™ Human Cytokine/Chemokine/Growth Factor Panel 1, 45plex
The ProcartaPlex Human Cytokine/Chemokine/Growth Factor Panel 1 45plex enables the exploration of immune function by analyzing 45 protein targets in a single well using Luminex xMAP technology.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Human IL-1 alpha ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 10% |
| Intraassay CV | 5.4 |
| Gene Symbol | IL-1 A,IL1,IL1-ALPHA,IL1F1 |
| Total Assay Time | 3 hrs 10 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 50 μL |
| Assay Sensitivity | 1.1 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 1.6 to 100 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 20 mins |
| Accession Number | P01583 |
| Regulatory Status | RUO |
| Target | IL-1 alpha |
| Kit Contents | Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3552 |
| Sample Type | Plasma,Serum,Supernatant,Urine |
| Label or Dye | HRP |
| Synonym | Hematopoietin 1, IL1F1, Interleukin 1 alpha |
Invitrogen™ Mouse Granzyme B Uncoated ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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R&D Systems™ Mouse IL-10 Quantikine ELISA Kit
Mouse IL-10 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
| Specificity | Natural and Recombinant Mouse IL-10 |
|---|---|
| Target Species | Mouse |
| Conjugate | HRP |
| Product Type | Solid Phase Sandwich ELISA |
| Gene ID (Entrez) | 16153 |
| Sample Type | Cell Culture Supernatant,Plasma (EDTA, Heparin),Serum |
| Synonym | CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL10A, IL-10 mgC126451, interleukin 10, interleukin-10, TGIF |
| For Use With (Application) | ELISA |
| Cross Reactivity | <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules |
Invitrogen™ Mouse IL-6 ELISA Kit, High Sensitivity
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet Ice |
|---|---|
| Immunoassay Kit Format | Sandwich ELISA Kit |
| Target Species | Mouse |
| Conjugate | HRP |
| Interassay CV | 11% |
| Intraassay CV | 8.8% |
| Gene Symbol | Il6 |
| Total Assay Time | 18 hr |
| Assay Sensitivity | 0.21 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 1.56-100 pg/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P08505 |
| Regulatory Status | RUO |
| Target | IL-6 |
| Kit Contents | Pre-coated microwell plate, Biotinylated Detection Antibody, Streptavidin-HRP, Standard, Sample Diluent, Assay Buffer, Amplification Reagent I, Amplification Reagent II, Wash Buffer Concentrate, Substrate Solution, Stop Solution, Adhesive Plate Covers |
| Gene Alias | B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6; |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Gene ID (Entrez) | 16193 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
Invitrogen™ Human GDF-8/Myostatin ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | Mstn |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.83 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.83 to 200 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | Cmpt; compact; GDF8; GDF-8; growth differentiation factor 8; growth/differentiation factor 8; H-Myo-Propeptide; H-Myostatin; MSLHP; MSTN; myostatin |
| Regulatory Status | RUO |
| Target | Myostatin |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 2660 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
Invitrogen™ Human TGF beta-1 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 4.9% |
| Intraassay CV | 3.2% |
| Total Assay Time | 4 hr 30 min |
| Sample Volume | Plasma 20 μL, Serum 20 μL, Supernatant 20 μL |
| Assay Sensitivity | 8.6 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 31-2000 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 20 min |
| Accession Number | P01137 |
| Regulatory Status | RUO |
| Target | TGF beta-1 |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 7040 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
| Synonym | Camurati-Engelmann disease, DPD1 |
Invitrogen™ Human IL-2 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Protein Aliases | IL-2, T-cell growth factor, TCGF |
|---|---|
| Immunoassay Kit Format | Sandwich ELISA Kit |
| Target Species | Human |
| Conjugate | HRP |
| Interassay CV | 5% |
| Intraassay CV | 7% |
| Gene Symbol | IL2 |
| Total Assay Time | 3 hr 30 min |
| Assay Sensitivity | 9.1 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 18.8-1200 pg/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P60568 |
| Regulatory Status | RUO |
| Target | IL-2 |
| Kit Contents | Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Controls, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Controls,Chromogen,Stop Solution,Adhesive Plate Covers |
| Gene Alias | aldesleukin; cytokine; H-IL-2; il 2; Il2; Il-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; interleukin 2 variant IL2delta2; Interleukin2; Interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; interleukin-2; interleukin 2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; patial sequence of Interleukin-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGFaldesleukin; cytokine; H-IL-2; il 2; Il2; Il-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; interleukin 2 variant IL2delta2; Interleukin2; Interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; interleukin-2; interleukin 2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; patial sequence of Interleukin-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF; IL-2; TCGF; lymphokine |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Gene ID (Entrez) | 3558 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
Invitrogen™ Human tPA ELISA Kit
ELISA
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 6.5% |
| Intraassay CV | 3.6 |
| Gene Symbol | T-PA,TPA |
| Total Assay Time | 2 hrs 10 mins |
| Sample Volume | Serum 10 μL, Supernatant 10 μL |
| Assay Sensitivity | 6 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 15.6 to 1,000 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 20 mins |
| Accession Number | P00750 |
| Regulatory Status | RUO |
| Target | tPA |
| Kit Contents | Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Controls,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 5327 |
| Sample Type | Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Tissue-type plasminogen activator |
Invitrogen™ Rat IL-6 ELISA Kit
ELISA
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| Target Species | Rat |
|---|---|
| Conjugate | HRP |
| Interassay CV | 10% |
| Intraassay CV | 5 |
| Gene Symbol | ILg6,Ifnb2 |
| Total Assay Time | 3 hrs 10 mins |
| Sample Volume | Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 12.0 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 31.3 to 2,000 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 20 mins |
| Accession Number | P20607 |
| Regulatory Status | RUO |
| Target | IL-6 |
| Kit Contents | Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 24498 |
| Sample Type | Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Interferon-beta 2, B-cell stimulatory factor 2,HGF |